Today we started off by taking a look at our plate that we inoculated with our bacteria and added antibiotics to. The antibiotics we used were Penicillin, cinnamon, lemon, Augmentin, (add two other antibiotics). We analyzed the size of the circles that it killed to measure its effectiveness against our bacteria. We found that the drug that our bacteria was most sensitive to was (TE 30, add real name), which had the largest radius of dead bacteria around it. But, on the whole, it was relatively resistant to all of the antibiotics we tested it against. It was really interesting to see how our bacteria reacts with different drugs, which helped us to ultimately narrow down which type of bacteria we had.
We also checked the results of our throat and nasal swabs, as well as our urine sample. The Blood agar for the throat swab was used to detect Streptococcus progenies or Streptococcus alactica. Our blood agar had gamma-hemolysis, meaning that there was no lysis of red blood cells and it was a negative test.
The mannitol plate used for the nasal swab was looking for MRSA, which would have a yellow color due to pH change. Pete’s nasal sample had some colonies growing, but obviously not yellow, so we know it’s not MRSA.
There was no growth on the EMB plate used to test the urine sample. All in all, we turned out decently healthy guys.
Next, we tried an food purity test (Antibody-Antigen Reaction in Agar). We did this by taking an agar plate and making four “wells” in it (poking holes in it with a pipet). In the center well, we placed a drop of Bovine Albumin, and in the surrounding wells, we placed a drop of Goat Anti-horse, Anti-Bovine, and Anti-swine albumin. We also made another agar that was exactly the same, except the center well had Hamburger extract instead of Bovine Albumin. This experiment will test the purity of the Bovine albumin and the Hamburger extract.
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